Date of Award
Andrew A. Yeagley
In the current methodology of fighting infections, antibiotics are used to eliminate the invading bacteria. A result of this process is constantly evolving antibiotic-resistant bacteria. If we hope to be more effective at preventing disease, we must shift the way we view microbial treatment. One novel method of preventing bacterial proliferation is the modulation of biofilm. Many bacteria can produce a biofilm; an extracellular substance that provides protection and antibiotic resistance to the bacteria. Attacking this biofilm and allowing the body’s natural defenses to clear out the bacteria is one promising way to treat bacterial infections while avoiding adaptive bacterial mutation.
Isatin has been shown to regulate biofilm and thus exhibits properties similar to quorum sensing molecules. Additionally, isatin derived from indole which is a known quorum sensing molecule. Previous studies have shown that hydrazone and oxime derivatives of isatin have antimicrobial properties, which make them targets of interest in testing the ability of isatins to regulate biofilms. Within this study, a library of these compounds was created and tested against E. coli, P. aeruginosa, S. aureus to determine the OD600 correlation, minimum inhibitory concentration and the effect the compounds have on biofilm formation. For E. coli it was found that a compound was found to inhibit while others promoted. For P. aeruginosa, it was found that a compound was found to inhibit biofilm production, while two others were found to be promoters. Compounds were found to be biofilm inhibitors for S. aureus, while a compound was found to be a promoter.
Nuckolls, Sarah E. and Yeagley, Andrew A., "The Evaluation of Isatin 3-Hydrazone Derivatives toward Biofilm Regulation" (2015). Theses & Honors Papers. 53.