Date of Award
David W. Buckalew. Ph.D.
Researchers often debate whether all serovars of Salmonella enterica are pathogenic to humans. Broiler houses containing chickens are a major source of Salmonella and serve as potential reservoirs. Therefore, farmers must take substantial measures in order to avoid contamination with their products. The primary objective of the study was to determine whether the four most clinically relevant Salmonella spp. serovars from poultry houses could be isolated from the environmental waterways in Prince Edward and Cumberland Counties of Virginia. The study adapted a serotyping method designed for typing Salmonella to gain a perspective of possible serovars that may be found in local waterways. All of the isolates tested positive in a latex solution to confirm the isolate as Salmonella spp presence. 15 out of the 32 isolates were typed to Group 1 Salmonella, which is most closely associated with human illness. Additionally, 13 isolates were able to be identified to one of the four targeted serovars. The four targeted serovars were Typhimurium, Enteritidis, Hadar, and Heidelberg. The study was not able to accurately serotype any of the aforementioned serovars.
Smith, Jr., Timothy M., "Identifying pathogenic Salmonella serotypes isolated from South Central VA waterways via sequential PCR analysis" (2013). Theses & Honors Papers. 144.